QIAGEN

REPLI-g Screening Kit

For high-throughput manual or automated whole genome amplification from small or precious samples

·         Process 96 samples with just 20 minutes of handling time

·         Parallel WGA of multiple samples

·         Highly-stable DNA suitable for long-term storage

·         Room temperature setup allows easy liquid handling

Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification).

The REPLI?g Screening Kit provides DNA polymerase, buffers, and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, blood, and cultured cells.

The streamlined, room-temperature procedure enables straightforward liquid handling that requires just 20 minutes.

The kit is designed for rapid mutation screening using a range of genotyping, PCR, sequencing, or microarray assays. Amplified DNA can be used directly in most downstream applications without purification or quantification.

Feature	Specifications
Amplification	Whole genomic DNA
Applications	Genotyping, sequencing
Denaturation step	Heat
Handling time for 96 samples	20 minutes
Maximum input volume	10 ng DNA, 0.5 µl whole blood, >600 cells/µl
Minimal pipetting volume needed	1 µl
Quality assessment	No
Reaction time	10-16 hours (overnight)
Reaction volume	40 µl
Samples per run; throughput	High (1-96 samples)
Starting amount of DNA	>10 ng purified genomic DNA
Starting material	Genomic DNA, blood, cells
Technology	Multiple Displacement Amplification (MDA)
Yield	~8 µg

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